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High-Performance Liquid Chromatography (HPLC)
High-Performance Liquid Chromatography (HPLC)
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The analytical chemistry method of high-performance liquid chromatography (HPLC) is used to separate, recognize, and quantify each component in combination. It uses pumps to move a column of solid adsorbent material through a pressured liquid solvent containing the sample combination. The adsorbent material and each component in the sample interact slightly differently, resulting in various flow rates for the different substances and their separation as they exit the column.